Otein (COMP) by an original sandwich ELISA with monoclonal antibodies 16-F
Otein (COMP) by an original sandwich ELISA with monoclonal antibodies 16-F 12 and 17 C 10 [2]. MMP-9, TIMP and YKL-40 were estimated by commercial ELISA kits. Joint space width was measured in the narrowest point of the tibiofemoral compartment by 0.1 caliper and magnifying glass. Results Eighty-nine patients with knee OA were included in the study and were followed for 2 years. The paired samples of serum and synovial fluid were obtained from 48 patients in second study. The joint space narrowing in 2 years was 0.4 ?0.79 mm. The patients with knee OA had higher initial serum values of Baicalein 6-methyl ether biological activity pentosidine than controls (P = 0.04) and also of TIMP (P = 0.04), MMP-9 (P = 0.02) and COMP (P = 0.05). The patients with initially higher serum levels of pentosidine had more rapid radiological progression than controls (r = 0.30), and the same was true for hyaluronic acid (r = 0.56). Serum pentosidine levels correlated with synovial fluid pentosidine levels (r2 = 0.78). Mild correlation occurred between synovial fluid pentosidine and COMP levels (r2 = 0.11, P < 0.05). Conclusions Serum pentosidine levels may be a new molecular marker for prediction of OA progression. References 1. Spacek P, Adam M: Pentosidin HPLC determination in body fluids and tissues as a marker glycation and oxidation loading of the organism in osteoarthritis. Osteoarthritis Cartil, in press. 2. Vil V, Ob ka Z, Vyt ek R, Senolt L, Tchetverikov I, Kraus VB, Pavelka K: Monoclonal antibodies to human cartilage oligomeric matrix protein (comp): epitope mapping and characterization of sandwich ELISA. Clin Chim Acta 2002, 8019:1-11. Acknowledgement Supported by grant NK/5366-4 IGA from the Ministry of Health Czech Republic.168 Exercise: medicine for knee cartilage?L Dahlberg1, E Roos2, J Svensson3, P Leander4, CJ Tiderius5 of Orthopedics, Malm?University PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27689333 Hospital, and Lund University, Sweden; 2Department of Orthopedics, University Hospital, Lund, and Lund University, Sweden; 3Department of Radiation Physics, Malm?University Hospital, Malm? Sweden; 4Department of Radiology, Malm?University Hospital, Malm? Sweden; 5Department of Orthopedics, Malm?University Hospital, Malm? Sweden Arthritis Res Ther 2003, 5(Suppl 3):168 (DOI 10.1186/ar969)1Department167 Pentosidine in serum and synovial fluid in patients with knee osteoarthritis and its potential role of prediction of osteoarthritis progressionK Pavelka, L Senolt, V Vilim, P Spacek, M Braun, S Forejtova Institute of Rheumatology, Prague 2, Czech Republic PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/26437915 Arthritis Res Ther 2003, 5(Suppl 3):167 (DOI 10.1186/ar968) Background The role of molecular markers for prediction of osteoarthritis (OA) progression is not yet defined. Pentosidine, one of the wellcharacterized advanced glycation endproducts, may be a candidate. Objectives To study the role of pentosidine as a marker of knee OA, as a marker of progression of knee OA, and for correlation of pentosidine in serum and synovial fluid and correlation with other markers.SMany of the 10?0 of the working-age population with knee pain will develop osteoarthritis (OA), a progressive joint disease with cartilage deterioration and increased disability. In knee OA, exercise decreases joint pain and improves function. Lack of human in vivo monitoring methods has made studies of influence of exercise on cartilage composition impossible. Delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) can estimate joint cartilage glycosaminoglycan (GAG) content. It is based on the principle.
