Ndogenous antinociceptive system was previously demonstrated in rainbow trout [20] and piaucu [21]. The existence of an ?endonegous antinociceptive system in fish suggests that this system evolved early in the vertebrate phylum because it is present in a basal vertebrate group, the fish. The existence of an endogenous antinociceptive system induced by restraint has been described in mammals and amphibians [33]?[37]. Its activation promotes the inhibition of nociceptive responses, as evaluated by the tail flick, hot plate, acetic acid and formaldehyde tests [33] 37]. In our 10457188 study, a short-termrestraint was sufficient to produce antinociception in fish. PZ-51 Although studies with mammals and amphibians use a longer period of restraint to induce antinociception (0.5 to 4 hours) [33]?[37], another study in mammals showed that this effect can also be observed a few minutes (5 min) after the start of the restraint [38]. In addition to presenting a rapid activation, the restraint promotes short-term antinociception in fish that lasts approximately 5 min. Studies on mammals show that the duration of the antinociception is variable depending on the type and intensity of the stress. In mammals, short-term antinociception was described in studies that used forced swimming [39], immobilized-water immersion [40], and various parameters of footshock [40],[41], with durations of 5 to 15 min. Long-term antinociception was promoted by a long period of restraint (30 min) [33] with a duration of more than 60 min. The short-term antinociception observed in the present study could be related to the short 1315463 period of restraint that was used in the experimental protocol. Longer periods of restraint could promote antinociception for a different duration. By analyzing the participation of the m-opioid receptor on the restraint -induced antinociception, using naloxone, we observed that this response seems to be mediated by distinct mechanisms in fish, depending on the duration of the stress. The antinociception promoted by 3 min of stress is blocked by naloxone, which suggests the participation of a preferential m-opioid mechanism, while the 5 min antinociception, not blocked by naloxone, suggests the participation of non-preferential m-opioid and/or non-opioid mechanisms. These results corroborate studies on mammals, which also show that stress-induced antinociception can be blocked or not by naloxone, depending on temporal and spatial Bexagliflozin biological activity factors [40] 47]. The activation of different mechanisms of nociceptive modulation in fish supports the idea that the process underlying nociception and antinociception in this group can be complex. There is evidence for the existence of a functional opioid system, with the presence of the opioid receptor m similar to mammalian receptors [15] and the inhibition of nociceptive-related behaviors by morphine and tramadol application [3], [5]. The activation of an opioid endogenous analgesic system was described in piaucu ?that were submitted to the conspecific alarm substance [21].Stress-Induced Antinociception in FishFurthermore, in rainbow trout, the stress promoted by one week of social subordination can activate an endogenous antinociceptive system [20]. However, this study did not evaluate the participation of the opioid system in this response. To our knowledge, the present study is the first to demonstrate the modulation of nociceptive behaviors by an endogenous opioid system in fish activated by a standard acute stressful stimulus.Ndogenous antinociceptive system was previously demonstrated in rainbow trout [20] and piaucu [21]. The existence of an ?endonegous antinociceptive system in fish suggests that this system evolved early in the vertebrate phylum because it is present in a basal vertebrate group, the fish. The existence of an endogenous antinociceptive system induced by restraint has been described in mammals and amphibians [33]?[37]. Its activation promotes the inhibition of nociceptive responses, as evaluated by the tail flick, hot plate, acetic acid and formaldehyde tests [33] 37]. In our 10457188 study, a short-termrestraint was sufficient to produce antinociception in fish. Although studies with mammals and amphibians use a longer period of restraint to induce antinociception (0.5 to 4 hours) [33]?[37], another study in mammals showed that this effect can also be observed a few minutes (5 min) after the start of the restraint [38]. In addition to presenting a rapid activation, the restraint promotes short-term antinociception in fish that lasts approximately 5 min. Studies on mammals show that the duration of the antinociception is variable depending on the type and intensity of the stress. In mammals, short-term antinociception was described in studies that used forced swimming [39], immobilized-water immersion [40], and various parameters of footshock [40],[41], with durations of 5 to 15 min. Long-term antinociception was promoted by a long period of restraint (30 min) [33] with a duration of more than 60 min. The short-term antinociception observed in the present study could be related to the short 1315463 period of restraint that was used in the experimental protocol. Longer periods of restraint could promote antinociception for a different duration. By analyzing the participation of the m-opioid receptor on the restraint -induced antinociception, using naloxone, we observed that this response seems to be mediated by distinct mechanisms in fish, depending on the duration of the stress. The antinociception promoted by 3 min of stress is blocked by naloxone, which suggests the participation of a preferential m-opioid mechanism, while the 5 min antinociception, not blocked by naloxone, suggests the participation of non-preferential m-opioid and/or non-opioid mechanisms. These results corroborate studies on mammals, which also show that stress-induced antinociception can be blocked or not by naloxone, depending on temporal and spatial factors [40] 47]. The activation of different mechanisms of nociceptive modulation in fish supports the idea that the process underlying nociception and antinociception in this group can be complex. There is evidence for the existence of a functional opioid system, with the presence of the opioid receptor m similar to mammalian receptors [15] and the inhibition of nociceptive-related behaviors by morphine and tramadol application [3], [5]. The activation of an opioid endogenous analgesic system was described in piaucu ?that were submitted to the conspecific alarm substance [21].Stress-Induced Antinociception in FishFurthermore, in rainbow trout, the stress promoted by one week of social subordination can activate an endogenous antinociceptive system [20]. However, this study did not evaluate the participation of the opioid system in this response. To our knowledge, the present study is the first to demonstrate the modulation of nociceptive behaviors by an endogenous opioid system in fish activated by a standard acute stressful stimulus.
