ALK/CD246 Antibody (5A4) Summary
| Immunogen |
Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus, corresponding to amino acids 419-520 of Human ALK.
|
| Localization |
Cell Membrane
|
| Specificity |
ALK (5A4)
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
ALK
|
| Purity |
Tissue culture supernatant
|
| Innovators Reward |
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
IHC-P: 1/25 – 1/70. An incubation period of 60 minutes at room temperature is recommendedc using an ABC method. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
|
Reactivity Notes
Cross-reacts with Human. Expected to cross-react with Mouse (85% identity with immunogen) due to sequence homology. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
No buffer
|
| Preservative |
0.09% Sodium Azide
|
| Purity |
Tissue culture supernatant
|
Alternate Names for ALK/CD246 Antibody (5A4)
- ALK tyrosine kinase receptor
- ALK
- anaplastic lymphoma kinase (Ki-1)
- Anaplastic lymphoma kinase
- anaplastic lymphoma receptor tyrosine kinase
- CD246 antigen
- CD246
- EC 2.7.10.1
- Ki-1
- NBLST3
- Tcrz
Background
Anaplastic lymphoma kinase (ALK) was originally discovered as a NPM (Nucleophosmin)-ALK fusion protein. ALK and LTK (leukocyte tyrosine kinase) are receptor tyrosine kinases similar to the insulin receptor subfamily of kinases. LTK is found in murine B Lymphocyte precursors and in forebrain neurons. ALK is usually found in the nervous system. A truncated form of ALK occurs in many non Hodgkins lymphomas. The ALK gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM ALK) resulting from t(2;5) translocation is a protein of 80kDa with the N terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SUDHL1 lymphoid cell lines strongly express NPM ALK.