AP2A2 Antibody Summary
| Immunogen |
Synthetic peptides corresponding to AP2A2 (adaptor-related protein complex 2, alpha 2 subunit) The peptide sequence was selected from the C terminal of AP2A2 (NP_036437). Peptide sequence LAKLKKKKGPSTVTDLEDTKRDRSVDVNGGPEPAPASTSAVSTPSPSADL.
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| Specificity |
This product is specific to Subunit or Isofrom: alpha-2.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
AP2A2
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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| Theoretical MW |
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
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| Buffer |
PBS and 2% Sucrose
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| Preservative |
0.09% Sodium Azide
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| Purity |
Immunogen affinity purified
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Notes
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Alternate Names for AP2A2 Antibody
- 100 kDa coated vesicle protein C
- Adapter-related protein complex 2 alpha-2 subunit
- adaptin, alpha B
- Adaptor protein complex AP-2 subunit alpha-2
- adaptor-related protein complex 2, alpha 2 subunit
- alpha2-adaptin
- Alpha-adaptin C
- alpha-adaptin C; Huntingtin interacting protein J
- AP-2 complex subunit alpha-2
- Clathrin assembly protein complex 2 alpha-C large chain
- clathrin-associated/assembly/adaptor protein, large, alpha 2
- DKFZP564D1864
- HIP9ADTAB
- Huntingtin yeast partner J
- Huntingtin-interacting protein 9
- Huntingtin-interacting protein J
- HYPJCLAPA2
- KIAA0899HIP-9
- Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
Background
AP2A2 is a component of the adaptor protein complex 2 (AP-2). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.