Product: Wilforine

Ankyrin 1 Antibody Summary

Immunogen
Synthetic peptides corresponding to ANK1(ankyrin 1, erythrocytic) The peptide sequence was selected from the middle region of ANK1 (NP_065210).Peptide sequence PCAMPETVVIRSEEQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTG.
Clonality
Polyclonal
Host
Rabbit
Gene
ANK1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/ml
Application Notes
This is a rabbit polyclonal antibody against ANK1 and was validated on Western blot.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Theoretical MW
189 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
From PBS & 2% Sucrose.
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Ankyrin 1 Antibody

  • ANK
  • ANK-1
  • ankyrin 1, erythrocytic
  • ankyrin-1
  • ankyrin-R
  • Erythrocyte ankyrin
  • SPH1
  • SPH2

Background

Ankyrins are a family of proteins that are believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of spec

PMID: 22216277

Product: Cot inhibitor-3

Ankyrin 1 Antibody Summary

Immunogen
Peptide with sequence C-QIVKRASLKRGKQ corresponding to C-Terminus according to NP_065208.2, NP_065209.2, NP_065210.2, NP_065211.2, NP_065213.2.
Epitope
C Terminus
Specificity
This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).
Clonality
Polyclonal
Host
Goat
Gene
ANK1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave approx. 19-23 kDa band bands in human skeletal muscle lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa band bands in the literature for this tissuescientific literature (PMID: 9430667) and given the calculated size of 17.6 kDa band according to NP_065211.2. The 19-22 kDa band bands were successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-02311.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at [email protected] for more details.

Alternate Names for Ankyrin 1 Antibody

  • ANK
  • ANK-1
  • ankyrin 1, erythrocytic
  • ankyrin-1
  • ankyrin-R
  • Erythrocyte ankyrin
  • SPH1
  • SPH2

PMID: 20695846