Apc4 Antibody (CIV1.1) Summary
| Immunogen |
C-terminus of APC4
|
| Specificity |
CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.
|
| Isotype |
IgG
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
ANAPC4
|
| Purity |
Protein G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1.0 mg/ml
|
| Purity |
Protein G purified
|
Notes
Positive control(s): Purified APC/C by silver and mass spectrometry.
Alternate Names for Apc4 Antibody (CIV1.1)
- anaphase promoting complex subunit 4
- anaphase-promoting complex subunit 4
- APC4Cyclosome subunit 4