Product: CHIR-99022 (trihydrochloride)
Apc4 Antibody (CIV1.1) [DyLight 350] Summary
| Immunogen |
C-terminus of APC4
|
| Specificity |
CIV1.1 may be used for the affinity purification of the Anaphase Promoting Complex (APC/C) by peptide elution. Suggested Dilution for western blot: 1:500. It is suggested to blot for at least 2 hours (wet transfer) to ensure that APC4 is transferred efficiently.Mass spectrometry was used to confirm that the whole APC/C is co-precipitated. To establish the APC4 specific bands, it is suggested to do an APC4 immunoprecipitation assay showing input, IP and FT and blot for at least 2 hours.
|
| Isotype |
IgG
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
ANAPC4
|
| Purity |
Protein G purified
|
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined.
|
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
|
| Buffer |
50mM Sodium Borate
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein G purified
|
Notes
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alternate Names for Apc4 Antibody (CIV1.1) [DyLight 350]
- anaphase promoting complex subunit 4
- anaphase-promoting complex subunit 4
- APC4Cyclosome subunit 4