CD177 Antibody (MEM-166) Summary
| Immunogen |
Human granulocytes
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| Specificity |
CD177 (MEM-166)
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| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
|
| Gene |
CD177
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| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter volume. It is recommended that the reagent be titrated for optimal performance for each application.
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| Positive Control |
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Reactivity Notes
Human, Expected Cross-Reactivity based on immunogen sequence: Rhesus.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS (pH 7.2)
|
| Preservative |
0.09% Sodium Azide
|
| Concentration |
0.5 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for CD177 Antibody (MEM-166)
- CD177 antigenNB1 GP
- CD177 molecule
- CD177
- HNA-2a
- HNA2Acell surface receptor
- Human neutrophil alloantigen 2a
- NB1 GP
- NB1PRV-1
- polycythemia rubra vera 1
- Polycythemia rubra vera protein 1
- PRV1
- PRV1NB1 glycoprotein
Background
CD177 is also known as neutrophil specific antigen 1, NB1, and polycythemia rubra vera 1. It is a member of the uPAR family and is a GPI-linked cell surface glycoprotein with a molecular weight of 60 kD. CD177 is expressed on granulocytes and bone marrow progenitors (early erythroblasts, megakaryocytes). It is thought to be involved in allogeneic and autoimmune responses to neutrophils. The MEM-166 antibody is useful for flow cytometry, immunoprecipitation, and Western blotting under non-reducing conditions.