CD30/TNFRSF8 Antibody (Ki-1/779)

Recombinant human TNFRSF8 protein

Cell surface

Hodgkin & Reed-Sternberg Cell Marker

Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkins disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This MAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).

IgG1 Kappa

Monoclonal

Mouse

TNFRSF8

Protein A or G purified

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Hu-chromosome location: 1p36.22
Molecular weight of antigen: 105-120kDa
Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 – 20 min followed by cooling at RT for 20 minutes.