DAPK3/ZIPK Antibody Summary
| Immunogen |
Rabbit anti-ZIP kinase (IN) polyclonal antibody was raised against a peptide corresponding to amino acids 279 to 298 of human origin (1).
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| Specificity |
ZIPK
|
| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
DAPK3
|
| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
This polyclonal antibody can be used for detection of ZIP kinase by Western blot at 1:1000 dilution. Jurkat or HeLa whole cell lysate can be used as positive control and a 52 kDa band should be detected. It has no cross response to DAP kinase. It is human, mouse, and rat reactive.
|
Reactivity Notes
This antibody is reactive against Human, Mouse, Rat
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS
|
| Preservative |
0.02% Sodium Azide
|
| Purity |
Immunogen affinity purified
|
Alternate Names for DAPK3/ZIPK Antibody
- DAP kinase 3
- DAPK3
- DAP-like kinase
- death-associated protein kinase 3
- Dlk
- EC 2.7.11
- EC 2.7.11.1
- ZIP kinase isoform
- ZIP
- ZIPK
- ZIPKFLJ36473
- ZIP-kinase
Background
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase (1). ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues (1).