DGCR8 [p Ser377] Antibody Summary
| Immunogen |
Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer377 of human DGCR8.
|
| Modification |
p Ser377
|
| Specificity |
Specific for the ~120k DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
|
| Preparation Method |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
|
| Gene |
DGCR8
|
| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
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Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
|
| Preservative |
No Preservative
|
| Purity |
Immunogen affinity purified
|
Alternate Names for DGCR8 [p Ser377] Antibody
- C22orf12
- DGCRK6chromosome 22 open reading frame 12
- DiGeorge syndrome critical region 8
- DiGeorge syndrome critical region gene 8
- Gy1
- microprocessor complex subunit DGCR8
- pasha
Background
The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han J et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller et al, 2010).