Product: APS-2-80 (hydrochloride)
Fc gamma RIIA/CD32a Antibody (7.3) [Alexa Fluor® 488] Summary
| Immunogen |
K562 and FcgRII+L cells
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| Localization |
Cell Surface
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| Specificity |
This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab2 fragments block immune complex binding. CD32 (Fc?RII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
|
| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
FCGR2A
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
Optimal dilution of this antibody shomld be experimentally determined.
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Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
|
| Buffer |
50mM Sodium Borate
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Notes
This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
Alternate Names for Fc gamma RIIA/CD32a Antibody (7.3) [Alexa Fluor® 488]
- CD32 antigen
- CD32a
- CD32MGC23887
- CDw32fc-gamma-RIIa
- Fc fragment of IgG, low affinity IIa, receptor (CD32)
- Fc fragment of IgG, low affinity IIa, receptor for (CD32)
- Fc gamma RIIA
- FCG2
- Fc-gamma RII-a
- Fc-gamma-RIIa
- FcGR
- FCGR2
- FCGR2A
- FCGR2A1
- FcgRIIA
- FCRIIA
- fcRII-a
- IGFR2MGC30032
- IgG Fc receptor II-a
- Immunoglobulin G Fc receptor II
- low affinity immunoglobulin gamma Fc region receptor II-a