Fc gamma RIIA/CD32a Antibody (7.3) [DyLight 650] Summary
| Immunogen |
K562 and FcgRII+L cells
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| Localization |
Cell Surface
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| Specificity |
This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab2 fragments block immune complex binding. CD32 (Fc?RII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
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| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
FCGR2A
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Optimal dilution of this antibody shomld be experimentally determined.
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Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
|
| Buffer |
50mM Sodium Borate
|
| Preservative |
0.05% Sodium Azide
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| Purity |
Protein A or G purified
|
Notes
Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Alternate Names for Fc gamma RIIA/CD32a Antibody (7.3) [DyLight 650]
- CD32 antigen
- CD32a
- CD32MGC23887
- CDw32fc-gamma-RIIa
- Fc fragment of IgG, low affinity IIa, receptor (CD32)
- Fc fragment of IgG, low affinity IIa, receptor for (CD32)
- Fc gamma RIIA
- FCG2
- Fc-gamma RII-a
- Fc-gamma-RIIa
- FcGR
- FCGR2
- FCGR2A
- FCGR2A1
- FcgRIIA
- FCRIIA
- fcRII-a
- IGFR2MGC30032
- IgG Fc receptor II-a
- Immunoglobulin G Fc receptor II
- low affinity immunoglobulin gamma Fc region receptor II-a