Hepatitis B Virus Antibody Summary
| Immunogen |
Synthetic peptide corresponding to the amino acids 21-47 of the native molecule conjugated to Keyhole limpet Haemocyanin.
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| Specificity |
Recognises the 39 and 41 kD (L-protein) in Blotting. Both bands are peptide blockable.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Purity |
Protein G purified
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Applications/Dilutions
| Dilutions |
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| Publications |
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Reactivity Notes
Viral. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS (pH 7.2)
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| Preservative |
0.1% Sodium Azide
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| Concentration |
5.0 mg/ml
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| Purity |
Protein G purified
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Alternate Names for Hepatitis B Virus Antibody
- HBsAg
- Hepatitis B Receptor Binding Fragment Pre S
Background
Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase.Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed.The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.