Human Spleen Whole Tissue Lysate (Adult Whole Normal) Summary
| Specificity |
Human Spleen Tissue Lysate
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Applications/Dilutions
| Application Notes |
This product can be used for: Western Blot (10 ug to 20 ug per lane is recommended for a mini gel), Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, protein-protein interaction, gel shift assay, tissue specific expression, and mass spec. analysis.
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
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| Buffer |
HEPES (pH7.9), KCl, Sucrose, Sodium deoxycholate, and NP-40, MagCl2, EDTA, Glycerol, and a cocktail of protease inhibitors.
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| Preservative |
No Preservative
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| Concentration |
5.0 mg/ml
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Lysate Details for Spleen
| Type |
Tissue
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| Life Stage |
Adult
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| Protein State |
Native
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| Tissue Condition |
Normal
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| Subcellular Fraction |
Whole
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Notes
Protein lysate is verified on SDS-PAGE comparing to standard protein lysate. Protein lysate can be validated by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.
Background
The identification of the human genome has promoted novel studies involving aging, behavioral research, diabetes and obesity, immunology, bioassay and pharmacological screening. Western blot analysis assists scientists conducting such research to make important contributions to functional genomics, allowing for structural, functional and comparative analysis of proteins in human models.