IL-27/IL-35 EBI3 Subunit Antibody Summary
| Immunogen |
This antibody was prepared by repeated immunizations with recombinant mouse EBI3.
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| Specificity |
Cross reactivity does NOT occur with human EBI-3. Cross-reactivity with IL-27 from other sources has not been determined.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
EBI3
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| Purity |
IgG purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting.Expect a band approximately 23 kDa in size corresponding – the mature mouse EBI3 protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.
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Reactivity Notes
Mouse, rat
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| Preservative |
0.01% Sodium Azide
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| Concentration |
LYOPH
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| Purity |
IgG purified
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| Reconstitution Instructions |
Reconstitute with 50 ul of distilled water to a final concentration of 1.0 mg/ml.
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Alternate Names for IL-27/IL-35 EBI3 Subunit Antibody
- cytokine receptor
- EBI3
- EBV-induced gene 3 protein
- Epstein-Barr virus induced 3
- Epstein-Barr virus induced gene 3
- Epstein-Barr virus-induced gene 3 protein
- IL-27 subunit beta
- IL-27/IL-35 EBI3 Subunit
- IL27B
- IL-27B
- IL35 EBI3
- interleukin-27 subunit beta
Background
The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses.
The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer.