Luciferase Antibody (LUC-Y) Summary
| Immunogen |
Recombinant luciferase expressed in E. coli.
|
| Specificity |
Detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Purity |
Protein A purified
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
For Flow Cytometry, membrane permeabilisation is required. For Flow Cytometry we recommend using 0.1ug per 1 x 10^6 cells.
|
Reactivity Notes
Reacts with Insect.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate buffered saline
|
| Preservative |
0.09% Sodium Azide
|
| Concentration |
1.0 mg/ml
|
| Purity |
Protein A purified
|
Alternate Names for Luciferase Antibody (LUC-Y)
- ec 1.13.12.7
- Fluc
- Luciferase
- luciferin 4 monooxygenase
Background
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg+2 and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.