OCT4 Antibody (NRG1.1) Summary
| Immunogen |
BALB/C mice injected with synthetic peptide of human OCT-4 origin.
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| Localization |
Nuclear
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| Marker |
Embryonic Stem Cell Marker
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| Specificity |
OCT-4 immunostaining has been shown to be a sensitive and specific marker for seminomatous / (dys)germinomatous tumours and in embryonal carcinoma variants of non-seminomatous GCTs, whether in primary gonadal or extragonadal sites or in metastatic lesions.
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| Isotype |
IgG2b
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
POU5F1
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
For IHC: Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
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| Positive Control |
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| Publications |
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
No buffer
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| Preservative |
0.05% Sodium Azide
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| Purity |
Protein A or G purified
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Alternate Names for OCT4 Antibody (NRG1.1)
- class 5, transcription factor 1
- MGC22487
- Oct-3
- OCT3Oct4
- Oct-4
- Octamer-binding protein 3
- otc-4
- OTF3POU domain class 5, transcription factor 1
- OTF4
- POU class 5 homeobox 1
- POU-type homeodomain-containing DNA-binding protein
Background
Members of Oct family of transcription factors specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation. Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells.