PKA R2 Overexpression Lysate (Denatured) Summary
| Description |
Pasmid: pCMV-PRKAR2A full-length
|
| Gene |
PRKAR2A
|
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).
|
Lysate Details for PKA R2
| Type |
Overexpression
|
| Protein State |
Denatured
|
Notes
Quality control test: Transient overexpression cell lysate was tested with Anti-PRKAR2A antibody by Western Blots.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for PKA R2 Overexpression Lysate (Denatured)
- protein kinase, cAMP-dependent, regulatory, type II, alpha
- RII-alpha subunit
Background
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq]