Product: MDL 105519

POLR3F Antibody Summary

Immunogen
Synthetic peptides corresponding to POLR3F(polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa) The peptide sequence was selected from the middle region of POLR3F.Peptide sequence LNQQCFKFLQSKAETARESKQNPMIQRNSSFASSHEVWKYICELGISKVE.
Specificity
This product is specific to Subunit or Isofrom: RPC6.
Clonality
Polyclonal
Host
Rabbit
Gene
POLR3F
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against POLR3F and was validated on Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for POLR3F Antibody

  • DNA-directed RNA polymerase III 39 kDa polypeptide
  • DNA-directed RNA polymerase III subunit F
  • DNA-directed RNA polymerase III subunit RPC6
  • DNA-directed RNA polymerases III 39 kDa polypeptide
  • polymerase (RNA) III (DNA directed) polypeptide F (39 kDa)
  • polymerase (RNA) III (DNA directed) polypeptide F, 39 kDa
  • RNA polymerase III 39 kDa subunit
  • RNA polymerase III C39 subunit
  • RNA polymerase III subunit C6
  • RPC39MGC13517
  • RPC6

Background

POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.

PMID: 21249195