Recombinant Bacteria TB Ab85A Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 43 to 338 of Mycobacterium tuberculosis TB Ab85A
Source: Baculovirus Amino Acid Sequence: ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH |
| Preparation Method |
Hi-5
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
fbpA
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
32.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
Mycobacterium tuberculosis
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol
|
| Concentration |
0.5 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 32.8kDa (305aa)
Alternate Names for Recombinant Bacteria TB Ab85A Protein
- 85 A
- 85A
- Ag85A
- Antigen 85 A
- Antigen 85 complex A
- Antigen 85A
- Fbp A
- FbpA
- Fibronectin Binding protein A
- Mpt 44
- Mpt44
- Mycobacterium tuberculosis antigen 85A
- Mycolyl transferase 85 A
- Mycolyl transferase 85A
- Rv3804c
- Secreted antigen Ag85A
Background
Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6,6-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.
