Product: Ticarcillin (disodium)
Recombinant Bacterial L-Asparaginase Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 338 of E.coli L-Asparaginase
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 39.3 kDa (358aa), confirmed by MALDI-TOF
|
| Theoretical MW |
39.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
E.coli
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Bacterial L-Asparaginase Protein
- AnsA
- AnsB
- Asparaginase
- Colaspase
- Cytoplasmic asparaginase I
- L ASNase I
- L ASNase II
- L asparaginase 1
- L asparaginase 2
- L asparaginase I
- L asparaginase II precursor
- L asparaginase II
- L asparagine amidohydrolase I
- L asparagine amidohydrolase II
Background
AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E.coli ansA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
