Recombinant Bacterial Uridine Phosphorylase 1/UPP1 Protein Summary
| Description |
A recombinant protein with a His tag and corresponding to amino acids 1 – 253 of UPP1.
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSKSDVFHLG LTKNDLQGAT LAIVPGDPDR VEKIAALMDK PVKLASHREF TTWRAELDGK PVIVCSTGIG GPSTSIAVEE LAQLGIRTFL RIGTTGAIQP HINVGDVLVT TASVRLDGAS LHFAPLEFPA VADFECTTAL VEAAKSIGAT THVGVTASSD TFYPGQERYD TYSGRVVRHF KGSMEEWQAM GVMNYEMESA TLLTMCASQG LRAGMVAGVI VNRTQQEIPN AETMKQTESH AVKIVVEAAR RLL |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity is > 20,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of uridine presence of phosphate per minute at pH 7.5 at 25C.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
UPP1
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 29.3kDa (273aa) confirmed by MALDI-TOF The His tag increases overall size to 273 residues.
|
| Theoretical MW |
29.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
This is an E. coli protein
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl
|
| Concentration |
1.0 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Bacterial Uridine Phosphorylase 1/UPP1 Protein
- EC 2.4.2.3
- UDPRASE
- UDRPASE
- UP
- UPASE 1
- UPASE
- UPP
- UPP1
- UPUPase 1
- UrdPase 1
- uridine phosphorylase 1
- uridine phosphorylase
Background
Uridine phosphorypase(UDP) catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. This enzyme belongs to the family of glycosyltransferases, specifically the pentosyltransferases. The systematic name of this enzyme class is uridine:phosphate alpha-D-ribosyltransferase. Other names in common use include pyrimidine phosphorylase, UrdPase, UPH, and UPase. Recombinant E.coli UDP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
