Product: 10-Undecenoic acid (zinc salt)
Recombinant Human Aldo-keto Reductase 1B10/AKR1B10 Protein Summary
| Description |
An un-tagged recombinant protein corresponding to amino acids 1 to 316 of Human Aldo-keto Reductase 1B10/AKR1B10
Source: E.coli Amino Acid Sequence: MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity is > 1,500pmol/min/ug, and was obtained by measuring the decrease of NADP in absorbance at 340nm resulting from NADPH at pH 7.0 at 37C.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
AKR1B10
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
Assay
1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50 ul of recombinant AKR1B10 solution with various concentrations (2.5ug, 5ug, 10ug) in 750 ul reaction buffer. 3. Mix by inversion and Incubate at 25C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes. |
| Theoretical MW |
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 36 kDa (316aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human Aldo-keto Reductase 1B10/AKR1B10 Protein
- AKR1B10
- AKR1B11
- AKR1B12
- Aldo-keto Reductase 1B10
- aldo-keto reductase family 1 member B10
- aldo-keto reductase family 1, member B10 (aldose reductase)
- aldo-keto reductase family 1, member B11 (aldose reductase-like)
- AldoketoReductase 1B10
- aldose reductase-like 1
- aldose reductase-like peptide
- Aldose reductase-like
- Aldose reductase-related protein
- ALDRLn
- ARL1
- ARL-1SI reductase
- ARP
- EC 1.1.1
- EC 1.1.1.-
- EC 1.1.1.21
- hARP
- HIS
- HSI
- MGC14103
- Small intestine reductase
Background
AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer. Recombinant human AKR1B10 protein was expressed in E.coli and purified by using conventional chromatography.
