Recombinant Human C4 binding protein B Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 18 to 252 of Human C4 binding protein B
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSDAEH CPELPPVDNS IFVAKEVEGQ ILGTYVCIKG YHLVGKKTLF CNASKEWDNT TTECRLGHCP DPVLVNGEFS SSGPVNVSDK ITFMCNDHYI LKGSNRSQCL EDHTWAPPFP ICKSRDCDPP GNPVHGYFEG NNFTLGSTIS YYCEDRYYLV GVQEQQCVDG EWSSALPVCK LIQEAPKPEC EKALLAFQES KNLCEAMENF MQQLKESGMT MEELKYSLEL KKAELKAKLL |
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
C4BPB
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
This product is useful for SDS-PAGE. The purity of this protein is > 90% by SDS – PAGE.
|
| Theoretical MW |
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.15M NaCl
|
| Concentration |
1 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Human C4 binding protein B Protein
- C4b binding protein, beta chain
- C4b-binding protein beta chain
- C4BP
- complement component 4 binding protein, beta chain
- complement component 4 binding protein, beta
- complement component 4-binding protein, beta
Background
C4BPB is a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain of this protein assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. Recombinant human C4BPB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
