Recombinant Human CBP20 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 156 of Human CBP20
Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNQ |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
NCBP2
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 20.1 kDa (176aa), confirmed by MALDI-TOF
|
| Theoretical MW |
20.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
|
| Concentration |
0.5 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Human CBP20 Protein
- 20 kDa nuclear cap-binding protein
- Cbc2
- CBP20CBC2
- NCBP 20 kDa subunit
- NCBP interacting protein 1
- NCBP-interacting protein 1
- NIP1Cell proliferation-inducing gene 55 protein
- nuclear cap binding protein subunit 2, 20kD
- nuclear cap binding protein subunit 2, 20kDa
- nuclear cap-binding protein subunit 2
Background
NCBP2, also known as CBC2, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5 cap of nascent pre-mRNA in the nucleoplasm. This protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Recombinant human NCBP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
