Recombinant Human HP1 alpha Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 191 of Human HP1 alpha
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGKKTK RTADSSSSED EEEYVVEKVL DRRVVKGQVE YLLKWKGFSE EHNTWEPEKN LDCPELISEF MKKYKKMKEG ENNKPREKSE SNKRKSNFSN SADDIKSKKK REQSNDIARG FERGLEPEKI IGATDSCGDL MFLMKWKDTD EADLVLAKEA NVKCPQIVIA FYEERLTWHA YPEDAENKEK ETAKS |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
CBX5
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 24.8 kDa (215aa), confirmed by MALDI-TOF
|
| Theoretical MW |
24.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
|
| Concentration |
1 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Human HP1 alpha Protein
- Antigen p25
- chromobox homolog 5 (Drosophila HP1 alpha)
- chromobox homolog 5 (HP1 alpha homolog, Drosophila)
- chromobox homolog 5
- chromobox protein homolog 5
- Heterochromatin protein 1 homolog alpha
- heterochromatin protein 1-alpha
- HP1 alpha homolog
- HP1 alpha
- HP1
- HP1A
- HP1-ALPHA
- HP1Hs alpha
- HP1Hs-alpha
Background
Chromobox homolog 5, also known as CBX5, is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. CBX5 is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. It has a pseudogene located on chromosome 3. Also, The CBX5 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated. Recombinant human CBX5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
