Recombinant Human IFN-gamma Protein Summary
| Description |
A recombinant protein corresponding to IFNG.
Amino Acid Sequence: MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFRGRRASQ |
| Preparation Method |
E.coli
|
| Details of Functionality |
Measured in a cytotoxicity assay using WiDr cells. The ED50 for this effect is less or equal to 5 ng/ml.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
IFNG
|
| Purity |
>95% pure by SDS-PAGE
|
| Endotoxin Note |
< 1.0 EU per 1 microgram of protein (determined by LAL method)
|
Applications/Dilutions
| Theoretical MW |
16.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate-buffered saline (pH 7.4), 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 16.9kDa (144aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human IFN-gamma Protein
- IFG
- IFI
- IFNG
- IFNgamma
- IFN-gamma
- Immune interferon
- interferon gamma
- interferon, gamma
Background
Nature human interferon-gamma is composed of 143amino acid residues without cysteine residues and is glycosylated. Recombinant human interferon-gamma was expressed in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Additional amino acid(Methionine) is attached at N-terminus.
