Product: Denatonium (benzoate)
Recombinant Human Leptin/OB Protein Summary
| Description |
A recombinant protein corresponding to LEP.
Source: E. coli |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
LEP
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 16 kDa (147 aa), confirmed by MALDI-TOF.
|
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate buffered saline, pH 7.4.
|
| Concentration |
1 mg/ml
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Human Leptin/OB Protein
- FLJ94114
- LEP
- leptin (murine obesity homolog)
- leptin (obesity homolog, mouse)
- Leptin
- OB
- Obese protein
- obese, mouse, homolog of
- Obesity factor
- OBOBS
Background
Human leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. The function of leptin in regulating appetite and metabolism, as well as the possibility of using leptin as a therapeutic agent, are currently under intense investigation. Recombinant human leptin was overexpressed as insoluble protein aggregate in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
