Product: Furaltadone (hydrochloride)
Recombinant Human Lipocalin-2/NGAL Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 21 to 198 of Human Lipocalin-2/NGAL
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MQDSTSDLIP APPLSKVPLQ QNFQDNQFQG KWYVVGLAGN AILREDKDPQ KMYATIYELK EDKSYNVTSV LFRKKKCDYW IRTFVPGCQP GEFTLGNIKS YPGLTSYLVR VVSTNYNQHA MVFFKKVSQN REYFKITLYG RTKELTSELK ENFIRFSKSL GLPENHIVFP VPIDQCIDG |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
LCN2
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 22.8kDa (199aa), confirmed by MALDI-TOF
|
| Theoretical MW |
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 50mM NaCl
|
| Concentration |
1 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Human Lipocalin-2/NGAL Protein
- 24p3
- 25 kDa alpha-2-microglobulin-related subunit of MMP-9
- HNL
- LCN2
- lipocalin 2 (oncogene 24p3)
- lipocalin 2
- Lipocalin2
- Lipocalin-2
- migration-stimulating factor inhibitor
- MSFI
- neutrophil gelatinase-associated lipocalin
- NGAL
- NGALlipocalin-2
- Oncogene 24p3
- p25
- Siderocalin
Background
Neutrophil gelatinase-associated lipocalin (NGAL), also known as LCN2, belongs to the calycin superfamily and Lipocalin family. LCN2 is an iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. The binding of LCN2 to bacterial siderophores is important in the innate immune response to bacterial infection. Also LCN2 functions as a growth factor. LCN2 is strongly upregulated during inflammation and is upregulated by interleukin 1 (but not TNF alpha) in humans. Recombinant human LCN2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
