Recombinant Human NMNAT-1 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 115-270 of Human NMNAT-1
Source: E. coli Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDAYKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQSIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAK |
| Specificity |
Specific activity is > 7,000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.
|
| Details of Functionality |
Specific activity is > 7,000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
NMNAT1
|
| Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
|
Applications/Dilutions
| Application Notes |
Molecular weight confirmed by MALDI-TOF.
|
| Theoretical MW |
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT, 1mM EDTA
|
| Preservative |
No Preservative
|
| Concentration |
1 mg/ml
|
| Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
|
Alternate Names for Recombinant Human NMNAT-1 Protein
- EC 2.7.7.1
- EC 2.7.7.18
- NaMN adenylyltransferase 1
- nicotinamide mononucleotide adenylyltransferase 1
- nicotinamide nucleotide adenylyltransferase 1
- nicotinamide nucleotide adenylyltransferase
- Nicotinate-nucleotide adenylyltransferase 1
- NMN adenylyltransferase 1
- NMNAT1
- NMNAT-1
- NMNATPNAT1
- PNAT1
- pyridine nucleotide adenylyltransferase 1
