Product: Chlorhexidine (dihydrochloride)
Recombinant Human Peroxiredoxin 4 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 38 to 271 of Human Peroxiredoxin 4
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDY RGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSG HTLRGLFIID DKGILRQITL NDLPVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity is >2,000 pmol/min/ug. Enzymatic activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25C for 1minute.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
PRDX4
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 28.8kDa (255aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human Peroxiredoxin 4 Protein
- Antioxidant enzyme AOE372
- AOE372
- AOE37-2PRX-4
- EC 1.11.1.15
- Peroxiredoxin 4
- Peroxiredoxin IV
- peroxiredoxin-4
- PRDX4
- Prx4
- prx-4
- prx-IV
- thioredoxin peroxidase (antioxidant enzyme)
- Thioredoxin peroxidase AO372
- Thioredoxin-dependent peroxide reductase A0372
Background
PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation. Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.
