Recombinant Human S100P Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 95 of Human S100P
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTELETAMGM IIDVFSRYSG SEGSTQTLTK GELKVLMEKE LPGFLQSGKD KDAVDKLLKD LDANGDAQVD FSEFIVFVAA ITSACHKYFE KAGLK |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
S100P
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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Reactivity Notes
This is a Human protein
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT, 50mM NaCl
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 12.6kDa (115aa), confirmed by MALDI-TOF Chromatin Immunoprecipitation was reported in scientific literature.
Alternate Names for Recombinant Human S100P Protein
- MIG9
- migration-inducing gene 9
- Protein S100-E
- S100 calcium binding protein P
- S100 calcium-binding protein Pprotein S100-P
- S100E
- S100P
Background
S100P is a Ca2+ binding protein that belongs to S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100P is involved in diverse biological functions but the exact role or mechanism of its action is still largely unknown. Upon binding of calcium ions S100P undergoes a conformational change that results in an exposure of a hydrophobic surface which allows the interaction with specific target proteins. Recombinant human S100P protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
