Recombinant Human TSG101 Protein Summary
| Description |
A recombinant protein with a His tag and corresponding to amino acids 1 – 145 of TSG101.
Source: E. coli Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
TSG101
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Theoretical MW |
20.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
This is a Human protein
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
0.5 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 20.7 kDa (181aa), confirmed by MALDI-TOF
Alternate Names for Recombinant Human TSG101 Protein
- ESCRT-I complex subunit TSG101
- TSG10
- tumor susceptibility gene 10
- tumor susceptibility gene 101 protein
- tumor susceptibility gene 101
- tumor susceptibility protein
- VPS23
Background
TSG101(Tumor susceptibility gene 101) protein belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. This protein contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. Mutations and alternative splicing in this protein occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. Recombinant human TSG101 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
