Product: 6-Acetamidohexanoic acid
Recombinant Human Thioredoxin Reductase 1/TRXR1 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 161 to 649 of Human Thioredoxin Reductase 1/TRXR1
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MYDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQI EAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGCCG |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity is > 15 units/mg, and was measured in a coupled assay with 5,5 -Dithiobis(2-nitrobenzoic acid) (DTNB) and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
TXNRD1
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 55.9 kDa(510aa), confirmed by MALDI-TOF
|
| Theoretical MW |
55.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20 mM Tris-HCl buffer (pH8.0) contain.ning 10% glycerol
|
| Concentration |
1 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Human Thioredoxin Reductase 1/TRXR1 Protein
- EC 1.8.1
- EC 1.8.1.9
- Gene associated with retinoic and IFN-induced mortality 12 protein
- Gene associated with retinoic and interferon-induced mortality 12 protein
- GRIM12
- GRIM-12
- GRIM-12TR1
- KDRF
- KM-102-derived reductase-like factor
- MGC9145
- oxidoreductase
- Thioredoxin Reductase 1
- thioredoxin reductase 1, cytoplasmic
- thioredoxin reductase GRIM-12
- Thioredoxin reductase TR1
- TR
- TR1
- TRXR1
- TRXRD1
- TXNR
- TXNRD1
- TXNRTRXR1
Background
TXNRD1, also known as Thioredoxin reductase 1, is a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. Inhibition of TXNRD1 activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases. Recombinant human TXNRD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
