Recombinant Human UbcH5a/UBE2D1 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 147 of Human UbcH5a/UBE2D1
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMALKRIQ KELSDLQRDP PAHCSAGPVG DDLFHWQATI MGPPDSAYQG GVFFLTVHFP TDYPFKPPKI AFTTKIYHPN INSNGSICLD ILRSQWSPAL TVSKVLLSIC SLLCDPNPDD PLVPDIAQIY KSDKEKYNRH AREWTQKYAM |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
UBE2D1
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 19.0 kDa (170aa), confirmed by MALDI-TOF
|
| Theoretical MW |
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Human UbcH5a/UBE2D1 Protein
- E2(17)KB1
- Stimulator of Fe transportUBC4/5 homolog
- UBC4/5
- UBC5A
- UbcH5
- UbcH5a
- UBCH5AEC 6.3.2.19
- UBCH5SFT
- UBE2D1
- Ubiquitin carrier protein D1
- ubiquitin-conjugating enzyme E2 D1
- Ubiquitin-conjugating enzyme E2(17)KB 1
- Ubiquitin-conjugating enzyme E2-17 kDa 1
- ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
- Ubiquitin-protein ligase D1
Background
UBE2D1 belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBE2D1 is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Recombinant human UBE2D1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
