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Product: Clofoctol

Recombinant Human WDR5 Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 334 of Human WDR5

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATEEKKPET EAARAQPTPS SSATQSKPTP VKPNYALKFT LAGHTKAVSS VKFSPNGEWL ASSSADKLIK IWGAYDGKFE KTISGHKLGI SDVAWSSDSN LLVSASDDKT LKIWDVSSGK CLKTLKGHSN YVFCCNFNPQ SNLIVSGSFD ESVRIWDVKT GKCLKTLPAH SDPVSAVHFN RDGSLIVSSS YDGLCRIWDT ASGQCLKTLI DDDNPPVSFV KFSPNGKYIL AATLDNTLKL WDYSKGKCLK TYTGHKNEKY CIFANFSVTG GKWIVSGSED NLVYIWNLQT KEIVQKLQGH TDVVISTACH PTENIIASAA LENDKTIKLW KSDC

Preparation
Method
E.coli
Protein/Peptide Type
Recombinant Protein
Gene
WDR5
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Application Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 38.8 kDa (354aa), confirmed by MALDI-TOF
Theoretical MW
38.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human WDR5 Protein

  • BIG3
  • BIG-3
  • BMP2-induced 3-kb gene protein
  • SWD3
  • SWD3, Set1c WD40 repeat protein, homolog
  • WD repeat domain 5
  • WD repeat-containing protein 5
  • WDR5
  • WD-repeat protein 5

Background

WDR5, also designated BMP-2-induced gene 3 kb or BIG-3, belongs to the family of WD-40 repeat proteins, and is essential for vertebrate development, Hox gene activation and global H3K4 trimethylation. This protein is expressed in osteoblasts, chondrocytes, osteocytes and marrow stromal cells. WDR5 may play a role in its function of accelerating osteoblast differentiation. Recombinant human WDR5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

PMID: 24159189

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Author: premierroofingandsidinginc