Recombinant Mouse PGK1 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids1-4170 of Mouse PGK1
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSLSNK LTLDKLDVKG KRVVMRVDFN VPMKNNQITN NQRIKAAVPS IKFCLDNGAK SVVLMSHLGR PDGVPMPDKY SLEPVAAELK SLLGKDVLFL KDCVGPEVEN ACANPAAGTV ILLENLRFHV EEEGKGKDAS GNKVKAEPAK IDAFRASLSK LGDVYVNDAF GTAHRAHSSM VGVNLPQKAG GFLMKKELNY FAKALESPER PFLAILGGAK VADKIQLINN MLDKVNEMII GGGMAFTFLK VLNNMEIGTS LYDEEGAKIV KDLMSKAEKN GVKITLPVDF VTADKFDENA KTGQATVASG IPAGWMGLDC GTESSKKYAE AVGRAKQIVW NGPVGVFEWE AFARGTKSLM DEVVKATSRG CITIIGGGDT ATCCAKWNTE DKVSHVSTGG GASLELLEGK VLPGVDALSN V |
| Specificity |
Specific activity: > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
|
| Details of Functionality |
Specific activity: > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
PGK1
|
| Purity |
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
|
Applications/Dilutions
| Application Notes |
Molecular weight confirmed by MALDI-TOF.
|
| Theoretical MW |
47.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate buffered saline (pH 7.4) containing 10% glycerol, 1mM DTT
|
| Preservative |
No Preservative
|
| Concentration |
1 mg/ml
|
| Purity |
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
|
Alternate Names for Recombinant Mouse PGK1 Protein
- Cell migration-inducing gene 10 protein
- EC 2.7.2.3
- MGC142128
- MGC8947
- MIG10
- PGK1
- PGKA
- PGKAMGC117307
- phosphoglycerate kinase 1
- Primer recognition protein 2
- PRP 2
- PRP2
