Recombinant Mouse S100A1 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 94 of mouse S100A1
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSELESAME TLINVFHAHS GKEGDKYKLS KKELKDLLQT ELSGFLDVQK DADAVDKVMK ELDENGDGEV DFKEYVVLVA ALTVACNNFF WETS |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
S100A1
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 12.6 kDa (114aa), confirmed by MALDI-TOF
|
| Theoretical MW |
12.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
|
| Purity |
>90%, by SDS-PAGE
|
Alternate Names for Recombinant Mouse S100A1 Protein
- protein S100-A1
- S100 alpha
- S100 calcium binding protein A1
- S100 calcium-binding protein A1S100
- S-100 protein alpha chain
- S-100 protein subunit alpha
- S100 protein, alpha polypeptide
- S100A
- S100A1
- S100-alpha
Background
S100 calcium binding protein A1, also known as S100A, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A1 is expressed by striated muscle, endothelium and chondrocytes. Intracellularly, S100A1 regulates cytosolic Ca levels, and is part of the Hsp70/Hsp90 mutlichaperone complex that protects against protein denaturation. Recombinant mouse S100A1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
