Recombinant Mouse S100A8 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 89 of mouse S100A8
Source: E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPSELEKALS NLIDVYHNYS NIQGNHHALY KNDFKKMVTT ECPQFVQNIN IENLFRELDI NSDNAINFEE FLAMVIKVGV ASHKDSHKE |
| Preparation Method |
E.coli
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
S100A8
|
| Purity |
>95% pure by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 12.4 kDa (109aa), confirmed by MALDI-TOF
|
| Theoretical MW |
12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris-HCl buffer (pH8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT
|
| Purity |
>95% pure by SDS-PAGE
|
Alternate Names for Recombinant Mouse S100A8 Protein
- 60B8AG
- CAGACP-10
- Calgranulin A
- calgranulin-A
- Calprotectin L1L subunit
- CFAG
- CFAGL1Ag
- CGLA
- Cystic fibrosis antigen
- Leukocyte L1 complex light chain
- MA387
- MIF
- Migration inhibitory factor-related protein 8
- MRP8
- MRP-8
- MRP8S100 calcium binding protein A8 (calgranulin A)
- NIF
- P8
- protein S100-A8
- S100 calcium binding protein A8
- S100 calcium-binding protein A8 (calgranulin A)
- S100 calcium-binding protein A8
- S100A8
- Urinary stone protein band A
Background
S100A8, also known MRP8, is a member of the S100 family, EF-hand superfamily of calcium binding proteins. This protein is localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Altered expression of this protein is associated with the disease cystic fibrosis. Recombinant mouse S100A8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
