SOD1/Cu-Zn SOD Antibody (2F5) Summary
| Immunogen |
Superoxide dismutase enzyme prepared from bovine erythrocytes.
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| Specificity |
NB100-65670 does not cross react with immunoglobulins but does react weakly with Mn SOD.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
SOD1
|
| Purity |
Protein G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
For IHC: This product does not require pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.
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Reactivity Notes
Reacts with Human. Cross reacts with Mouse and Bovine. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS, 0.1% BSA
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Protein G purified
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Alternate Names for SOD1/Cu-Zn SOD Antibody (2F5)
- ALS
- ALS1
- amyotrophic lateral sclerosis 1 (adult)
- Cu
- Cu/Zn superoxide dismutase
- CuZn SOD
- Cu-Zn SOD
- EC 1.15.1.1
- homodimer
- hSod1
- indophenoloxidase A
- Ipo1
- IPOA
- SOD
- SOD, cytosolic
- SOD, Soluble
- SOD1
- superoxide dismutase [Cu-Zn]
- Superoxide dismutase 1
- superoxide dismutase 1, soluble
- Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic
Background
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperon for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.Superoxide dismutase recognizes copper/zinc superoxide dismutase (SOD). Superoxide dismutases are ubiquitous metalloproteins that destroy oxygen-mediated free radicals, that are normally produced within the cells, which are toxic to biological systems. There are three forms of superoxide dismutase, including the Fe, mitochondrial (Mn) and the copper/zinc binding (Cu/Zn) form. The Cu/Zn form of SOD is utilised by most eukaryotic organisms. SOD Cu/Zn prevents oxygen-mediated free radical damage by catalysing the dismutation of the toxic superoxide radical to molecular oxygen and hydrogen peroxide.