Spectrin alpha 1 Antibody (AF10) Summary
| Immunogen |
The antibody reacts with the Erythroid alpha-Spectrin antigen.
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| Specificity |
The antibody is specific to the 240kD erythroid a-spectrin.
|
| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
SPTA1
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Various immunochemical reactions to detect erythroid spectrins. Identification of erythroid leukemias (1). Works in immunoprecipitation, western blotting and immunocytochemistry on frozen sections.Working dilution in western blotting is at least 1:1000.
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Reactivity Notes
Human
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
PBS solution containing 1.0% w/v BSA
|
| Preservative |
0.1% Sodium Azide
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| Concentration |
0.1 mg/ml
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| Purity |
Protein A or G purified
|
Alternate Names for Spectrin alpha 1 Antibody (AF10)
- alpha-I spectrin
- EL2
- erythrocyte
- HS3
- spectrin, alpha, erythrocytic 1 (elliptocytosis 2)
Background
Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface and transmembrane movement of phospholipids and cholesterol. On SDS PAGE gels erythrocyte spectrin appears as two bands referred to as alpha (240 kDa) and beta (220 kDa). While the simplest form of spectrin in solution is an alpha-beta heterodimer, spectrin can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of spectrin-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.