TDG Overexpression Lysate (Denatured) Summary
| Description |
Pasmid: pCMV-TDG full-length
|
| Gene |
TDG
|
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, and 0.01% Bromophenol blue).
|
Lysate Details for TDG
| Type |
Overexpression
|
| Protein State |
Denatured
|
Notes
Quality control test: Transient overexpression cell lysate was tested with Anti-TDG antibody by Western Blots.
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for TDG Overexpression Lysate (Denatured)
- EC 3.2.2.29
- TDG
- thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase
Background
The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]