VEGF-D Antibody (RM0007-8C35) Summary
| Immunogen |
This antibody was produced from a hybridoma (mouse myeloma fused withspleen cells from a rat immunized with purified mouse VEGF-D recombinant protein. IgG fraction of the culture supernatant was purified by Protein G affinity chromatography.
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| Specificity |
VEGFD (RM0007-8C35)
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| Isotype |
IgG2
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| Clonality |
Monoclonal
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| Host |
Rat
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| Gene |
FIGF
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| Purity |
Protein G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody recognizes mouse VEGF-D in western blot. No cross reactivity with mouse VEGF and VEGF-B.
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Reactivity Notes
This antibody is reactive against mouse. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
A 0.2 um filtered solution in PBS
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| Preservative |
No Preservative
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| Concentration |
LYOPH
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| Purity |
Protein G purified
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| Reconstitution Instructions |
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.
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Alternate Names for VEGF-D Antibody (RM0007-8C35)
- c-fos induced growth factor (vascular endothelial growth factor D)
- FIGF
- vascular endothelial growth factor D
- VEGFD
- VEGF-D
- VEGF-DVEGFDc-Fos-induced growth factor
Background
Vascular Endothelial Growth Factor D (VEGFD), also known as c-fos-induced growth factor (FIGF), is a member of the VEGF family of growth factors. Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFD is most closely related to VEGFC (23.3% amino acid sequence identity) and has a similar VEGF homology domain that spans the middle third of the precursor protein and the long N-terminal and C-terminal extensions. In adults, VEGFD is highly expressed in lung, heart, muscle, and small intestine. VEGFD expression in fibroblasts is induced by cell interaction mediated by cadherin 11. Recombinant human VEGFD is a ligand for the tyrosine kinases, VEGFR2 (Flk1) and VEGF receptor 3 (Flt4). Since VEGFR3 is strongly expressed in lymphatic endothelial cells, it is postulated that VEGFD is involved in the regulation of the growth and/or differentiation of lymphatic endothelium and thus, a mitogen for endothelial cells.