Ne Expression in the Liver of the African Lungfishmolluscs [35,63,64]. Secondly, it could be due to an increase in the turnover of free and bound iron as a result of the increase in synthesis of certain type of hemoglobins and/or hemoglobin in general. Delaney et al. [65] reported that 4 electrophoretically distinct types of hemoglobins (fraction I, II, III and IV) were present in P. aethiopicus, and there were increases in the amounts of types II and IV hemoglobins during the maintenance phase of aestivation. Hence, it is logical to deduce that changes in hemoglobin types during the induction phase of aestivation must be purchase Q-VD-OPh reverted back to normal during arousal, which could be one of the reasons that led to the up-regulation in mRNA expressions of transferrin and ferritin in the liver of P. annectens.Arousal phase: up-regulation of glutathione S-transferase (gst)GSTs are a major group of detoxification proteins involved in protecting against various reactive chemicals, including chemical carcinogens, secondary metabolites during oxidative stress, and chemotherapeutic agents [66]. They catalyze the reaction of glutathione with electrophilic centers of organic compounds [67]. These glutathione-conjugated compounds are rendered more water-soluble and more readily excreted. Besides, some GSTs have secondary catalytic activities including steroid isomerisation [68] and a selenium-independent peroxidase activity with organic hydroperoxides [69]. The alpha class GST (GSTa) may also function as intracellular transporters of various hydrophobic compounds (which are not substrates of GSTs) like bilirubin, heme, thyroid hormones, bile salts and steroids [70]. The increase in mRNA expression of gst in the liver of P. annectens after 1 day of arousal (Table 4) is indicative of a possible increase in secondary metabolites of oxidative stress and/or transport of heme in the liver. Similarly, increases in activity of Gst have been observed in aestivating snails and snails aroused from aestivation [71].Arousal phase: increase in SP600125 structure protein turnoverBased on the variety of genes related to protein synthesis, transport and folding in the forward and reverse library, it can be concluded that there was a high rate of protein turnover in the liver of lungfish after 1 day of arousal. It would appear that the machinery (e.g. ribosomal protein L12, L17 and L19) involved in the maintenance of protein structure during the maintenance phase (Table 4) was different from that (e.g. eIF4E-binding protein, eukaryotic translation elongation factor alpha 1 and elongation factor-1, delta b) involved in the regeneration of protein structure during the arousal phase (Table 5).ConclusionSix months of aestivation led to changes in gene expression related to nitrogen metabolism, oxidative defense, blood coagulation, complement fixation, iron and copper metabolism, and protein synthesis in liver of P. annectens. These results indicate that sustaining a low rate of waste production and conservation of energy store were essential to the maintenance phase of aestivation. On the other hand, there were changes in gene expression related to nitrogen metabolism, lipid metabolism, fatty acid transport, electron transport system, and ATP synthesis in liver of P. annectens after 1 day of arousal from 6 months of aestivation. It would appear that the freshly aroused fish depended on internal energy store for repair and structural modification. Overall, our results indicate that aestivation ca.Ne Expression in the Liver of the African Lungfishmolluscs [35,63,64]. Secondly, it could be due to an increase in the turnover of free and bound iron as a result of the increase in synthesis of certain type of hemoglobins and/or hemoglobin in general. Delaney et al. [65] reported that 4 electrophoretically distinct types of hemoglobins (fraction I, II, III and IV) were present in P. aethiopicus, and there were increases in the amounts of types II and IV hemoglobins during the maintenance phase of aestivation. Hence, it is logical to deduce that changes in hemoglobin types during the induction phase of aestivation must be reverted back to normal during arousal, which could be one of the reasons that led to the up-regulation in mRNA expressions of transferrin and ferritin in the liver of P. annectens.Arousal phase: up-regulation of glutathione S-transferase (gst)GSTs are a major group of detoxification proteins involved in protecting against various reactive chemicals, including chemical carcinogens, secondary metabolites during oxidative stress, and chemotherapeutic agents [66]. They catalyze the reaction of glutathione with electrophilic centers of organic compounds [67]. These glutathione-conjugated compounds are rendered more water-soluble and more readily excreted. Besides, some GSTs have secondary catalytic activities including steroid isomerisation [68] and a selenium-independent peroxidase activity with organic hydroperoxides [69]. The alpha class GST (GSTa) may also function as intracellular transporters of various hydrophobic compounds (which are not substrates of GSTs) like bilirubin, heme, thyroid hormones, bile salts and steroids [70]. The increase in mRNA expression of gst in the liver of P. annectens after 1 day of arousal (Table 4) is indicative of a possible increase in secondary metabolites of oxidative stress and/or transport of heme in the liver. Similarly, increases in activity of Gst have been observed in aestivating snails and snails aroused from aestivation [71].Arousal phase: increase in protein turnoverBased on the variety of genes related to protein synthesis, transport and folding in the forward and reverse library, it can be concluded that there was a high rate of protein turnover in the liver of lungfish after 1 day of arousal. It would appear that the machinery (e.g. ribosomal protein L12, L17 and L19) involved in the maintenance of protein structure during the maintenance phase (Table 4) was different from that (e.g. eIF4E-binding protein, eukaryotic translation elongation factor alpha 1 and elongation factor-1, delta b) involved in the regeneration of protein structure during the arousal phase (Table 5).ConclusionSix months of aestivation led to changes in gene expression related to nitrogen metabolism, oxidative defense, blood coagulation, complement fixation, iron and copper metabolism, and protein synthesis in liver of P. annectens. These results indicate that sustaining a low rate of waste production and conservation of energy store were essential to the maintenance phase of aestivation. On the other hand, there were changes in gene expression related to nitrogen metabolism, lipid metabolism, fatty acid transport, electron transport system, and ATP synthesis in liver of P. annectens after 1 day of arousal from 6 months of aestivation. It would appear that the freshly aroused fish depended on internal energy store for repair and structural modification. Overall, our results indicate that aestivation ca.
