L count, and the live sperm price, and enhanced the sperm abnormality rate. The hematological results obtained in the present study show drastically decreased testosterone levels in rats treated with paracetamol, indicating that the paracetamol could bring about testicular toxicity and impaired fertility. Equivalent final results were obtained by Luangpirom et al. [3] in male mice. This suggests that a higher dose of paracetamol (0.05 g/100 g b.wt) induces testicular toxicity. A substantial reduction in blood testosterone and impaired seminal quality have been found within the paracetamol group. Another study investigated the toxic effects of a high dose of paracetamol around the reproductive technique of male rabbits and showed comparable final results to these obtained in the present study. A considerable decrease in blood testosterone and seminal excellent impairment were observed within the group given high repeated therapeutic doses of paracetamol, which induced quite a few modifications and harmfully effected the histological structure from the seminiferous tubules. The study suggests that paracetamol can potentially lead to reproductive toxicity and needs to be utilised cautiously, specially when higher prolonged doses are indicated [29]. Additionally, the current study shows that paracetamol significantly decreased the erythrocyte (RBC) count, hemoglobin (Hb) content material, and packed cell volume (PCV). This reduce in RBCs and Hb reduces the oxygen-carrying capacity with the RBCs and decreases the quantity of oxygen reaching the cells, potentially causing anemia and impairing physique functions. Comparable reports show that treating rats with paracetamol decreased the PCV and RBC counts when compared with the controls [4,30]. Furthermore, the WBC and stab cell counts substantially enhanced in the paracetamol group when compared with the manage as well as other treatmentBiology 2021, 10,12 ofgroups. The present hematological outcomes suggest that overdoses and long-term treatment with paracetamol may perhaps stimulate the immune system to defend the body from infections. The existing outcomes are consistent having a study published in 2015 [30], which investigated the effect of paracetamol around the hematological parameters in rats. The rats were provided overdoses (300mg) of paracetamol for two days, as well as the outcomes showed significantly improved WBC counts within the treatment groups in comparison with the Monomethyl Epigenetic Reader Domain handle group. A polyphenol in EVOO is deemed to exhibit high antioxidant activity [31,32]. Thus, the present study investigated the protective impact of EVOO against paracetamol-induced hematotoxicity and testicular toxicity. The results obtained show amelioration inside the paracetamol-with-EVOO group compared with the paracetamol group. The histological and ultrastructural examination show that the majority of the seminiferous tubules contained normal spermatogonia, main spermatocytes, and spermatids. Sertoli cells had flattened nuclei and the sperm showed elongated condensed nuclei and acrosomal caps at the fronts on the heads. Spermatids appeared to have regular nuclei, and vacuoles were observed in some cells. The EVOO group showed improvements in a lot of the seminiferous tubules and much less prominent histopathological alterations when compared with the paracetamol group. This amelioration could be as a consequence of a polyphenolic element in EVOO in addition to a potential indirect reduction of oxidative tension by way of gene expression modulation and enzyme activity, which enhances enzymatic antioxidant defenses [16]. A related report [29] indicated that olive oil includes a protective effect against oxi.
