Seeds are visible, sheath open Seed formation V V Green/brown spathe, dark brown seeds are visible, sheath openFigure two. Figure two. Morphology in the 5 sexual reproductive stages (I-V)(I ) determined towards the repro- reproducMorphology of your 5 sexual reproductive stages determined to study study the ductive Methyl jasmonate supplier phenology of the Z. noltei meadows in the Ria de Aveiro lagoon. tive phenology in the Z. noltei meadows in the Ria de Aveiro lagoon.The total quantity of spathes collected in each reproductive stage during the study period was calculated to describe the basic reproductive phenology of Z. noltei in the To test the germination ability of your Z. noltei meadows, all collected sexual Ria de Aveiro lagoon. To test differences among meadows in the reproductive phenology, spathes, regardless of their stage in every single reproductive stage had been separated per meadowdate. sampling the percentage of spathes of sexual reproduction, was estimated by meadow and and2.3. Germination Abilitydate and cultured in aquaria (ten cm of diameter, filled with 600 mL artificial seawater) till 2.three. Germination Ability mature seeds have been obtained to apply for germination tests. To test the germination potential of was noltei meadows, 72 h to the mean seawater The salinity in each aquariumthe Z.adjusted just about every all collected sexual spathes, salinity regardless of theirZ. noltei meadow in the corresponding sampling date (Table S1). Aquaria obtained in each and every stage of sexual reproduction, were separated per meadow and sampling date and cultured in aquaria (ten cm of diameter, filled with 600 mL artificial seawere aerated (Air 550 R Plus, Sera GmbH, Heinsberg, Germany; O2 flux five mg/L) and water) till mature seeds have been obtained to apply for germination tests. maintained at area temperature (from 18.35 0.20 C to 23.66 0.12 C) under all-natural The salinity in each and every aquarium was adjusted every single 72 h for the imply seawater salinity photoperiod (from 14:10 to 12:12 the corresponding sampling date2019 toS1). Aquaria light/dark hours, from July (Table March 2020). Every single obtained in each Z. noltei meadow in week, aerated (Air 550 with tough, dark brown seed coat, have been O2 flux five counted and stored had been mature seeds, R Plus, Sera GmbH, Heinsberg, Germany; collected, mg/L) and at salinitiesat room 33 psu in 50 mL tubes illed to 23.66 0.12seawater to avoid natural maintained above temperature (from 18.35 0.20 with artificial ) under all-natural seed germination before the test. The seeds have been kept stored in the tubes till March 2020, when the germination tests have been carried out, coinciding together with the beginning of spring and also the all-natural germination period from the species [29]. Spathes that suffered senescence have been removed in the aquaria to prevent their decomposition. To stop overgrowth of epiphytes, artificial seawater was renewed, and the aquaria and aeration PHA-543613 Autophagy system had been cleaned once a week. Seed production was expressed because the variety of seeds per spathe. Germination tests were performed by exposing mature seeds to a low salinity shock to induce germination [12]. Seeds were introduced into aerated tubes (50 mL) filled withPlants 2021, ten,5 of20 mL of 1 psu artificial seawater and 20 mL of agar (five at 1 psu) as neutral substrate and placed inside an environmental chamber with controlled temperature and photoperiod (25.00 1.026 C, 12:12 light/dark hours, respectively). Then, the amount of germinated seeds (with broken seed coat and visible cotyledon) was recorded each week for 4 weeks to ca.
