Served 15 amino sequence momotif that’s lysine-rich (EKKGIMDKIKEKLPG), that is referred to as
Served 15 amino sequence momotif which is lysine-rich (EKKGIMDKIKEKLPG), which can be called the K-segment; a Y-segtif that is lysine-rich (EKKGIMDKIKEKLPG), that is known as the K-segment; a Y-segment ment situated N terminus ([T/V]D[E/Q]YGNP); and an an S-(serine-track repeats) motif Tianeptine sodium salt MedChemExpress located in thein the N terminus ([T/V]D[E/Q]YGNP); andS-(serine-track repeats) motif [3]. [3]. K-segment is viewed as the the segment of group II LEA proteins; it’s an an extenThe The K-segment is regarded corecore segment of group II LEA proteins; it isextensive sive segment and lays or much more a lot more repeats, developing amphiphilic -helixes in the Csegment and lays in onein a single orrepeats, making amphiphilic -helixes at the C-terminal terminal end of the[26]. In relation torelation to the arrangement and replication of these finish of your DNQX disodium salt iGluR proteins proteins [26]. Inside the arrangement and replication of those conserved conserved motif group II LEA proteins are classified classified into five subcategories: motif sequences, sequences, group II LEA proteins are into 5 subcategories: Kn, KnS, Kn, KnS, YnKn, YnSKn [30]. YnKn, SKn, and SKn, and YnSKn [30]. The proteins that possess only a a K-segment in their structural sequence belongthe proteins that possess only K-segment in their structural sequence belong to to the K-subgroup of group II LEA proteins, andSK-subgroup comprises those group II LEA K-subgroup of group II LEA proteins, and also the the SK-subgroup comprises those group II LEA proteins include an S-segment accompanied by a K-segment in their their sequences proteins that that contain an S-segment accompanied by a K-segment in sequences (Fig(Figure 1) [3]. A conserved segment of group group II LEA proteins was identified at the ure 1) [3]. A new new conserved segment of II LEA proteins was found at the N-termiN-terminus (DRGLFDFLGKK), which was termed the F-segment [31]. It was recently nus (DRGLFDFLGKK), which was termed the F-segment [31]. It was recently determined determined in plants as an overlooked motif of group II LEA proteins that hasfunctional in plants as an overlooked motif of group II LEA proteins that has possible potential functional properties of binding to membranes protein molecules molecules [31]. properties of binding to membranes as well as other and other protein [31].Figure 1. Schematic representation with the positions of repeated sequences that differentiate group II Figure 1. Schematic representation from the positions of repeated sequences that differentiate group II LEA protein subgroups. The blocks represent the arrangement the motifs inside the corresponding LEA protein subgroups. The blocks represent the arrangement ofof the motifs inside the corresponding subgroups of group II LEA proteins. The numbers on the ideal indicate the tandem repeats of subgroups of group II LEA proteins. The numbers on the ideal indicate the tandem repeats of each motif in diverse subgroups. The size range of group II LEA proteins is indicated at the top as the number of amino acid (aa) residues.The K-segments of group II LEA proteins interact with membranes and also other proteins to modulate the proteins’ phase properties and conformational transitions [32]. The K-Biomolecules 2021, 11,5 ofsegment happens in one particular to eleven copies inside a chain of amino acids [33]. It was reported in a study that the wheat group II LEA protein, DHN-5, shielded the activities of -glucosidase and lactate dehydrogenase (LDH) in vitro because of the presence of a K-segment in its amino acid.
