Rogression in inheritedThe Journal of Clinical Investigationhttp://www.jci.orgVolumeNumberNovember
moleculesArticleSIKVAV-Modified Chitosan Hydrogel as a Skin Substitutes for Wound Closure in MiceXionglin Chen 1, , Xiaoming Cao 2 , He Jiang 1 , Xiangxin Che two, , Xiaoyuan Xu 1 , Baicheng Ma 1 , Jie Zhang 1 and Tao HuangDepartment of Growth Differentiation Factor 1 (GDF-1) Proteins Species Histology Embryology and Medical Genetics, School of Fundamental Medical Sciences, Jiujiang University, Jiujiang 332000, China; [email protected] (H.J.); [email protected] (X.X.); [email protected] (B.M.); [email protected] (J.Z.); [email protected] (T.H.) Department of Anatomy, School of Simple Medical Sciences, Jiujiang University, Jiujiang 332000, China; [email protected] Correspondence: [email protected] (X.C.); [email protected] (X.C.); Tel.: +86-07928570078 (X.C.); +86-07928566041 (X.C.); Fax: +86-07928570078 (X.C.); +8607928566041 (X.C.)Received: two September 2018; Accepted: 9 October 2018; Published: 11 OctoberAbstract: Skin wound healing is often a complex and dynamic process that requires angiogenesis and growth factor secretion. Newly formed vessels can supply Cadherin-13 Proteins Purity & Documentation nutrition and oxygen for skin wound healing. Development aspects in skin wounds are crucial for keratinocytes and fibroblasts proliferation, epithelialization, extracellular matrix remodeling, and angiogenesis, which accelerate skin wound healing. Therefore, treatment approaches that boost angiogenesis and growth variables secretion in skin wounds can accelerate skin wound healing. This study investigated the effects of a SIKVAV (Ser-Ile-Lys-Val-Ala-Val) peptide-modified chitosan hydrogel on skin wound healing. Hematoxylin and eosin (H E) staining demonstrated that the SIKVAV-modified chitosan hydrogel accelerated the re-epithelialization of wounds compared with that observed within the negative and good controls. Masson’s trichrome staining showed that far more collagen fibers had been deposited in the skin wounds treated with the SIKVAV-modified chitosan hydrogel than within the damaging and constructive controls. Immunohistochemistry assays demonstrated that far more myofibroblasts have been deposited and much more angiogenesis occurred in skin wounds treated together with the SIKVAV-modified chitosan hydrogel than in the unfavorable and good controls. Also, ELISA assays showed that the SIKVAV-modified chitosan hydrogels promoted the secretion of development factors in skin wounds. Taken with each other, these results recommend that the SIKVAV-modified chitosan hydrogel has the potential to become created as synthesized biomaterials for the treatment of skin wounds. Key phrases: SIKVAV; chitosan hydrogel; skin wound healing; angiogenesis; growth factor1. Introduction The skin could be the biggest organ on the human physique, acting as a protective barrier against external disturbances. Intact skin prevents pathogen intrusion, infection, water, and electrolyte loss, and regulates the physique temperature [1,2]. Large-area full-thickness skin defects can cause severe clinical difficulties which include serious infections and shock caused by substantial water and electrolytes loss, which can potentially outcome in death. Skin wound healing can be a complicated and dynamic method that includes coagulation, inflammation, angiogenesis, epithelial regeneration, granulation tissue formation, extracellular matrix (ECM) deposition, and tissue remodeling [3]. For that reason, a comprehensive understanding with the mechanism of skin wound healing is essential to establish by far the most productive therapy [4]. The existing skin wound treatment approaches include things like c.
