Idin-treated). For For transcriptomic final results (values in bold), differentially expressed genes are indicated (FDR transcriptomic results (values in bold), differentially expressed genes are indicated (FDR 0.01). For 0.01). For proteomic benefits (values in italics), spots displaying a considerable difference in protein proteomic final results (values in (Studentspots SphK2 Inhibitor custom synthesis showing a substantial distinction in protein ratio values ratio values -1.25 or 1.25 italics), T-Test) are indicated. The grey location indicates proteins/tran-1.25 for 1.25 (Student T-Test) areobserved, while the blue location indicates a clothianidin impact. scripts or which a DMSO effect was indicated. The grey area indicates proteins/transcripts for which numbers outdoors of observed, diagram will be the total numbers clothianidin impact. proteins for Blue a DMSO impact was the Venn whilst the blue area indicates a of transcripts and Blue numbers outdoors of the Venn diagram would be the following clothianidin exposureproteins for which the expression which the expression is modified total numbers of transcripts and without the DMSO impact. is modified following clothianidin NTR1 Agonist web exposure without the need of the DMSO effect.three.three. Clothianidin Exposure Impacts Protein Biosynthesis and Metabolic Processes 3.3. Clothianidin Exposure Affects Protein Biosynthesis and Metabolic Processes In order to determine cellular processes associated to clothianidin exposure only, we conIn order to determine cellular processes associated expression modified following the closidered the transcripts and proteins that had theirto clothianidin exposure only, we thought of the transcripts and proteins that had their expression modified following the thianidin exposure (in blue, Figure three) and excluded transcripts and proteins showing a clothianidin exposure (in blue, Figure 3) and excluded transcripts and proteins displaying a DMSO effect (in grey, Figure three). By performing so, 1229 transcripts and 49 protein spots had been DMSO effect (in grey, Figure 3). By carrying out so, 1229 transcripts and 49 protein spots have been identified and regarded differentially expressed in relation with clothianidin exposure identified and regarded as differentially expressed in relation with clothianidin exposure (in blue, Figure 3). (in blue, Figure 3). Among these 1229 deregulated transcripts upon clothianidin exposure, 707 were upAmong these 1229 deregulated transcripts upon clothianidin exposure, 707 have been upregulated when 522 have been downregulated in comparison with both controls (handle and DMSO, regulated while 522 were downregulated compared to both controls (manage and DMSO, Table S3). Gene ontology enrichment analyses were performed on each categories of tranTable S3). Gene ontology enrichment analyses had been performed on both categories of scripts and revealed that clothianidin exposure led to (1) the downregulation of translatranscripts and revealed that clothianidin exposure led to (1) the downregulation of transtion (GO categories “translation”, “ribosome biogenesis”, “ribonucleoprotein complex aslation (GO categories “translation”, “ribosome biogenesis”, “ribonucleoprotein complex sembly” also as “small molecule metabolic processes” and “generation of precursor assembly” as well as “small molecule metabolic processes” and “generation of precursor metabolites and energy”) (Figure and to towards the upregulation of of genes involved in parmetabolites and energy”) (Figure four) 4) and (2)(two) the upregulationgenes involved in particuticular in two categories which might be relevant study: “signal.
